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	Workshops, seminars, hands-0n workshops in your lab, lectures and more are an essential part of the training and consulting provided by Proteomics.Asia. The hands-on training in your lab with your equipment by Proteomics.Asia cover these areas: Protein and Peptide analytics and purification Development of analysis strategies for recombinant proteins and synthetic peptides Identification of contaminants and by-products Characterization of disulfide-bridges and PTMs Lab-scale development of purification strategies Proteomics Cell culture and metabolomic labelling Lysis of cells and tissues Targeting subcellular components, membrane proteins, nuclear proteins QC of lysis Separation of proteins Chromatographic and electrophoretic separation techniques Immuno-affinity enrichment techniques Digestion of proteins for LC-MS/MS analysis Sample preparation for digestion In-gel digestion In-solution digestion Other enzymes than Trypsin Separation of peptides Chromatographic and electrophoretic separation techniques Orthogonal separation techniques High/low pH reversed-phase separations HILIC, ERLIC, IEX, and more Enrichment of PTMs Phosphorylations Glycosylations Many others from the known 600+ PTMs Proteomics of body fluids Serum, urine, saliva, CSF, and others Depletion and enrichment techniques Quantitaive mass spectrometry Metabolomic labelling of proteins Chemical labelling of peptides Labe-free techniques LC-MS/LC-MS/MS Set-up of a nano- or capillary-flow LC-MS/MS system Selection of the appropriate columns Optimising separation and MS-detection Optimising MS/MS parameters QC of the system Reducing carry-over Data recording and processing Data strorage and retrival, backup strategies Targeted mass spectrometry using MRM/SRM methods Development of MRM/SRM assays for protein and PTM identification and quantification Analysis of labelled peptides Database searches Use of the available software tools for database searches Selection of the appropriate search parameters Processing and reporting of database search results Validation of database search results Turning data into information Developing MRM/SRM assays to verify protein and PTM identifications Quantitative analysis of samples Other hands-on trainings Identification of interacting proteins Chemical proteomics Metabolomics Affinity-based techniques and beyond

Workshops, seminars, hands-0n workshops in your lab, lectures and more are an essential part of the training and consulting provided by Proteomics.Asia.